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Studies of proteins from one organism in another organism’s cells have shown that such exogenous proteins stick more, pointing toward coevolution of the cytoplasm and protein surface to minimize stickiness. Here we flip this question around by asking whether exogenous proteins can assemble efficiently into their target complexes in a non-native cytoplasm. We use as our model system the assembly of BtubA and BtubB from Prosthecobacter hosted in human U-2 OS cells. BtubA and B evolved from eukaryotic tubulins after horizontal gene transfer, but they have low surface sequence identity with the homologous human tubulins and do not respond to tubulin drugs such as nocodazole. In U-2 OS cells, BtubA and B assemble efficiently into dimers compared to in vitro, and the wild-type BtubA and B proteins subsequently are able to form microtubules as well. We find that generic crowding effects (Ficoll 70 in vitro) contribute significantly to efficient dimer assembly when compared to sticking interactions (U-2 OS cell lysate in vitro), consistent with the notion that a generic mechanism such as crowding can be effective at driving assembly of exogenous proteins, even when protein-cytoplasm quinary structure and sticking have been modified in a non-native cytoplasm. A simple Monte Carlo model of in vitro and in-cell interactions, treating BtubA and B as sticky dipoles in a matrix of sticky or nonsticky crowders, rationalizes all the experimental trends with two adjustable parameters and reveals nucleation as the likely mechanism for the time-scale separation between dimer- and tubule formation in-cell and in vitro. 

We present cosmological constraints from a gravitational lensing mass map covering 9400 deg²reconstructed from measurements of the cosmic microwave background (CMB) made by the Atacama Cosmology Telescope (ACT) from 2017 to 2021. In combination with measurements of baryon acoustic oscillations and big bang nucleosynthesis, we obtain the clustering amplitudeσ₈= 0.819 ± 0.015 at 1.8% precision,$S_8\equiv {\sigma }_8{({\mathrm{\Omega }}_{\mathrm{m}}/0.3)}^{0.5}=0.840\pm 0.028$, and the Hubble constantH₀= (68.3 ± 1.1) km s⁻¹Mpc⁻¹at 1.6% precision. A joint constraint with Planck CMB lensing yieldsσ₈= 0.812 ± 0.013,$S_8\equiv {\sigma }_8{({\mathrm{\Omega }}_{\mathrm{m}}/0.3)}^{0.5}=0.831\pm 0.023$, andH₀= (68.1 ± 1.0) km s⁻¹Mpc⁻¹. These measurements agree with ΛCDM extrapolations from the CMB anisotropies measured by Planck. We revisit constraints from the KiDS, DES, and HSC galaxy surveys with a uniform set of assumptions and find thatS₈from all three are lower than that from ACT+Planck lensing by levels ranging from 1.7σto 2.1σ. This motivates further measurements and comparison, not just between the CMB anisotropies and galaxy lensing but also between CMB lensing probingz∼ 0.5–5 on mostly linear scales and galaxy lensing atz∼ 0.5 on smaller scales. We combine with CMB anisotropies to constrain extensions of ΛCDM, limiting neutrino masses to ∑m_ν< 0.13 eV (95% c.l.), for example. We describe the mass map and related data products that will enable a wide array of cross-correlation science. Our results provide independent confirmation that the universe is spatially flat, conforms with general relativity, and is described remarkably well by the ΛCDM model, while paving a promising path for neutrino physics with lensing from upcoming ground-based CMB surveys.

 

We present new measurements of cosmic microwave background (CMB) lensing over 9400 deg²of the sky. These lensing measurements are derived from the Atacama Cosmology Telescope (ACT) Data Release 6 (DR6) CMB data set, which consists of five seasons of ACT CMB temperature and polarization observations. We determine the amplitude of the CMB lensing power spectrum at 2.3% precision (43σsignificance) using a novel pipeline that minimizes sensitivity to foregrounds and to noise properties. To ensure that our results are robust, we analyze an extensive set of null tests, consistency tests, and systematic error estimates and employ a blinded analysis framework. Our CMB lensing power spectrum measurement provides constraints on the amplitude of cosmic structure that do not depend on Planck or galaxy survey data, thus giving independent information about large-scale structure growth and potential tensions in structure measurements. The baseline spectrum is well fit by a lensing amplitude ofA_lens= 1.013 ± 0.023 relative to the Planck 2018 CMB power spectra best-fit ΛCDM model andA_lens= 1.005 ± 0.023 relative to the ACT DR4 + WMAP best-fit model. From our lensing power spectrum measurement, we derive constraints on the parameter combination$S_8^{\mathrm{CMBL}}\equiv {\sigma }_8{\left({\mathrm{\Omega }}_m/0.3\right)}^{0.25}$of$S_8^{\mathrm{CMBL}}=0.818\pm 0.022$from ACT DR6 CMB lensing alone and$S_8^{\mathrm{CMBL}}=0.813\pm 0.018$when combining ACT DR6 and PlanckNPIPECMB lensing power spectra. These results are in excellent agreement with ΛCDM model constraints from Planck or ACT DR4 + WMAP CMB power spectrum measurements. Our lensing measurements from redshiftsz∼ 0.5–5 are thus fully consistent with ΛCDM structure growth predictions based on CMB anisotropies probing primarilyz∼ 1100. We find no evidence for a suppression of the amplitude of cosmic structure at low redshifts.